Movie S5.

The nucleus of a primary hippocampal neuron expressing Halo-SRF was recorded with the ITM protocol. Several long-bound chromatin associated SRF molecules are visible. In ITM, 2× 50 ms exposures were followed by a 2s timelapse.

Single-molecule imaging of the transcription factor SRF reveals prolonged chromatin-binding kinetics upon cell stimulation

Lisa Hipp, Judith Beer, Oliver Kuchler, Matthias Reisser, Daniela Sinske, Jens Michaelis, J. Christof M. Gebhardt, and Bernd Knöll

PNAS. 2018. 116:880-889 DOI: 10.1073/pnas.1812734116