Movie S17.

Circumferential movement of single molecules of FtsW-HT (red) on septa of Spn cells (bright-field outlines; blue border). SM-TIRFm was performed on strain IU15096 (ftsW-ht) pre-labeled with a limiting amount of HT-JF549 ligand as described in SI Appendix, Experimental Procedures. Images were taken at 1 s intervals over the course of 180 s. Movie is shown at 15 frames per s. See analysis in Fig. 7F.