Movie S4.

Live-cell imaging of Golgi dispersion driven by a dimeric kinesin-1 motor. COS-7 cells expressing the dimeric kinesin-1 motor KIF5C(1-559)-mNG-FRB (green) and the peroxisome-targeted module PEX3-mRFP-FKBP (red) were imaged live for 2 min and then rapamycin was added (+RAP) and imaging was continued for 19 min. The cyan line indicates the nucleus and the tan line indicates the cell periphery. Scale bar: 10 μm.