Movie S1.

Movie of a 3.2-Å resolution cryo-EM map and model of VacA hexameric assembly (OA-1). Each of the 6 protomers is colored and annotated as (a,b,c,d,e, and f). The C-alpha backbone trace of a protomer made up of domain p33 and p55 are shown with the interaction residues of two adjacent protomers.