Movie S3.

Silencing and photorescue of mGlu₅ receptors by alloswitch and 1PE in acute brain slices. Video of OGB-1 fluorescence in a P9 rat acute brain slice, corresponding to images and Ca²⁺ traces in Fig. 5B–D in main text. Intracellular Ca²⁺ events are recorded at baseline and different drug and photostimulation conditions indicated by labels (‘baseline’ = aCSF containing TTX/LY367385; ‘+ alloswitch/no light’ = 10 μM alloswitch supplemented in recirculating aCSF; white dot indicates site and time of agonist pressure-ejection: DHPG, 1 mM and 800 hPa, 1 s; ‘1PE’ = 405 nm laser diode, 58 μW, 120 raster scans at 0.3 Hz) (SI Appendix, Materials and Methods 5.2). OGB-1 fluorescence is excited by raster scanning with a 488 nm Argon laser (2.4 μW, 1 Hz frame rate). Frame rate for OGB-1 is 0.3 Hz during photostimulation. Picture size is 350×350 μm.