Movie S4.

Photorescue of mGlu₅ receptors by 2PE in acute brain slices. Video of OGB-1 fluorescence in a P10 rat acute brain slice, corresponding to Ca²⁺ traces in Fig. 6B. Intracellular Ca²⁺ events are recorded in the dark and under photostimulation conditions indicated by labels (‘+ alloswitch/no light’ = 10 μM alloswitch supplemented in bubbling reservoir; ‘2PE’ = 780 nm pulsed laser, 25 mW, 120 raster scans at 0.3 Hz) (SI Appendix, Materials and Methods 5.2). The white dot indicates the site and time of agonist pressure-ejection (DHPG, 1 mM; 500 hPa, 1 s). OGB-1 fluorescence is excited by raster scanning with a 488 nm Argon laser (2.4 μW, 1 Hz frame rate). Frame rate for OGB-1 is 0.2 Hz during photostimulation. Picture size is 310×310 μm.