Movie S1.

Kinetochores retain Mps1 when tracking with disassembling microtubule tips. A kinetochore particle (from SBY15285) tracks with the disassembling tip of a microtubule (green) while carrying Mps1-SNAP-549 (cyan) and Mtw1-CLIP-647 (red). Colors are deliberately offset; the cyan-red pair is a colocalized, dual-color kinetochore particle.

Autophosphorylation is sufficient to release Mps1 kinase from native kinetochores

Lori B. Koch, Kwaku N. Opoku, Yi Deng, Adrienne Barber, Aimee J. Littleton, Nitobe London, Sue Biggins, and Charles L. Asbury

PNAS. 2019. 116:17355-17360 DOI: 10.1073/pnas.1901653116