Movie S5.

Real-time TCR-pMHC N4 tension during the migration of a OT-1 cell. A 12-min timelapse of RICM and TIRF showing a naïve OT-1 cell crawling over the substrate and producing transient tension when dimeric ICAM-1 was co-presented with pMHC N4. Note the TCR tension was highly dynamic and mostly located at the trailing edge. Scale bar = 10 μm.