Movie S10.
Tracked movie of neutrophil motility in the head mesenchyme of 3 dpf Tg(lyzC:miR-199-dendra2) zebrafish transiently injected with Tol2-lyzC-mCherry-2a-cdk2 WT. The video shows the motility of neutrophils expressing miR-199 alone or with Cdk2 expression. Videos were recorded for 30min with 1min interval. Neutrophils were grouped into single (green tracks) and double positive (red tracks) based on the presence of the mCherry reporter (Cdk2 WT). Representative videos from n = 4 independent experiments with 3 fish each group are shown. Scale bar: 100 μm.
Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration
PNAS. 2019. 116:18561-18570 DOI: 10.1073/pnas.1905221116