Movie S3.

Cell moving under 800 Pa compressive load and 0.5% agarose gel. An aggregation competent Ax2 cell expressing ABD-GFP as a reporter for F-actin, is chemotaxing to cyclic-AMP under 0.5% agarose and a compressive load of 400 Pa. The cell typically migrates with blebs (indicated by *) and infrequently with pseudopods. Taken by a laser scanning confocal microscope (Zeiss 780) at 2 frames per second about 8-10 minutes after the load is applied.