Movie S4.

R26E01>TrpA1 vs. Canton-S females at 29°C. Female flies showed “territorial behavior.” R26E01>TrpA1 would charge and head butt its wild type opponent until the opponent retreats. Once the fly retreats R26E01>TrpA1 remains on the food source.

A small number of cholinergic neurons mediate hyperaggression in female Drosophila

Caroline B. Palavicino-Maggio, Yick-Bun Chan, Claire McKellar, and Edward A. Kravitz

PNAS. 2019. 116:17029-17038 DOI: 10.1073/pnas.1907042116