Movie S5.

Three-dimensional visualization of the N-DRC from wild type, labeled DRC5-SNAP rescue and the difference map between them in longitudinal bottom views. Animated 3D visualization compares the isosurface renderings of the nexin-dynein regulatory complex (N-DRC) structures from wild-type (left top) and labeled DRC5-SNAP (left bottom) axonemes. The proximal ends of the flagella are on the left. The difference map shows the location of the SNAP-biotin-strep-Au label (yellow) at the C-terminus of DRC5 on the proximal side of the N-DRC linker. Compare with Fig. 7.