An anchovy (AF04 from Table S1) demonstrates a characteristic c-start escape response away from the stimulus when startled. High-speed cameras (250 Hz) allowed precise determination of the timing of response, and the frame number in the corner of the playback screen showed the stage of the animation at which the response occurred. In the overhead view the stimulus playback screen is situated along the top of the frame, the thick line was added to this image to represent the middle of the tank (16 cm from the screen), and the dotted lines represent the screen distance range (± 3 cm) at which a fish’s response must occur to be counted in our analysis.