Movie S4.

CCAP neurons sufficient to induce a Proboscis Extension Response (PER). Male CCAP-GAL4>UAS-ReaChR flies (7-9 days old) were photo-stimulated using a 620 nm LED, 10 pulses of 500 ms photo-stimulation at 0.2 Hz were delivered. The video shows one pulse train for one replicate (5 flies). Immediately after the first photo-stimulations some of the flies can be seem to extend their proboscis, this becomes even more evident after the second stimulation. The flies also become more hyperactive (leg movement) after each stimulation.