Movie S3.

(Related to Figure 4). CD8 T cells induce caspase activation in virus infected target cells. MEFs were infected with MCMV-3D.ΔM36 at an MOI of 0.2, 24 hours prior to co-culture with CD8 T cells (CD44+CD62L-) sorted from spleen of BL/6 mice latently infected with MCMV at 4:1 (E:T). Red florescent signal represent the reporter virus signal and green corresponds to CellEventTM Caspase-3/7 Green labelled apoptotic cells. Time represent hours post T cell co-culture.

Cytomegalovirus inhibition of extrinsic apoptosis determines fitness and resistance to cytotoxic CD8 T cells

M. Zeeshan Chaudhry, Rosaely Casalegno-Garduno, Katarzyna M. Sitnik, Bahram Kasmapour, Ann-Kathrin Pulm, Ilija Brizic, Britta Eiz-Vesper, Andreas Moosmann, Stipan Jonjic, Edward S. Mocarski, and Luka Cicin-Sain

PNAS. 2020. 117:12961-12968 DOI: 10.1073/pnas.1914667117