Movie S2.

Combined bioluminescence-fluorescence time-lapse microscopy of human pancreatic islets from T2D donors. Human α- and β-cells within islets from T2D donors were labeled, imaged and analyzed as described in the legend to Movie S1. Islets were synchronized by a 1 h forskolin pulse (time point 0), and subsequently subjected to bioluminescence-fluorescence time-lapse microscopy for at least 70 h. Upper left video shows bioluminescence acquisition; upper right video is a merge of bioluminescence channel (blue), green fluorescence channel (RIPGFP expression), and red fluorescence channel (Pppg-mCherry expression). Lower videos represent a close-up of one islet from the corresponding upper videos (the enlarged area is indicated with the blue square on the video).

In pancreatic islets from type 2 diabetes patients, the dampened circadian oscillators lead to reduced insulin and glucagon exocytosis

Volodymyr Petrenko, Nikhil R. Gandasi, Daniel Sage, Anders Tengholm, Sebastian Barg, and Charna Dibner

PNAS. 2020. 117:2484-2495 DOI: 10.1073/pnas.1916539117