Combined bioluminescence-fluorescence time-lapse microscopy of human pancreatic islets from T2D donors. Human α- and β-cells within islets from T2D donors were labeled, imaged and analyzed as described in the legend to Movie S1. Islets were synchronized by a 1 h forskolin pulse (time point 0), and subsequently subjected to bioluminescence-fluorescence time-lapse microscopy for at least 70 h. Upper left video shows bioluminescence acquisition; upper right video is a merge of bioluminescence channel (blue), green fluorescence channel (RIPGFP expression), and red fluorescence channel (Pppg-mCherry expression). Lower videos represent a close-up of one islet from the corresponding upper videos (the enlarged area is indicated with the blue square on the video).