Movie S3.

High-resolution keyframe image stacks of a selected region of E. chaffeensis–infected DH82 macrophages by FIB-SEM. The selected region of interest (red box in Video S2) was segmented at high resolution. Data were binned by 3 in the xy plane to give a final voxel size of 15 × 15 × 15 nm. Sequential stacks of 2D images were converted computationally to produce the video.

Host membrane lipids are trafficked to membranes of intravacuolar bacterium Ehrlichia chaffeensis

Mingqun Lin, Giovanna Grandinetti, Lisa M. Hartnell, Donald Bliss, Sriram Subramaniam, and Yasuko Rikihisa

PNAS. 2020. 117:8032-8043 DOI: 10.1073/pnas.1921619117