Movie S1.

Lysosomal TRPML1 activation results in mitochondrial calcium influx. Example time-lapse video of mitochondrial calcium response in live HeLa cells transfected with mitochondria matrix-targeted calcium sensor, Mito-R-GECO1, upon TRPML1 activation with ML-SA1 (31.25 μM; right) or vehicle control (left), corresponding to Fig. 1C. Scale bar, 10 μm.