Movie S4.

Transfer of calcium at mitochondria-lysosome contact sites in live HCT116 cells. Example time-lapse video of transfer of calcium (white arrow) to mitochondria following lysosomal TRPML1 activation with ML-SA1 (31.25 μM) at mitochondria-lysosome contacts in live HCT116 cells transfected with mitochondria matrix-targeted calcium sensor, Mito-R-GECO1, and lysosome marker, lamp1-mGFP, corresponding to Fig. S2C. Scale bar, 1 μm.