Colocalization of Flag-Orf6 with the NPC protein Nup98. HEK293T cells ectopically expressing Flag-Orf6 were analyzed by indirect immunofluorescence using anti-Flag (green) and anti-Nup98 (red) antibodies. Series of z-stack images obtained using a DeltaVision microscope are shown as movies. Merged images are shown in the left most panel.
Lisa Miorin, Thomas Kehrer, Maria Teresa Sanchez-Aparicio, Ke Zhang, Phillip Cohen, Roosheel S. Patel, Anastasija Cupic, Tadashi Makio, Menghan Mei, Elena Moreno, Oded Danziger, Kris M. White, Raveen Rathnasinghe, Melissa Uccellini, Shengyan Gao, Teresa Aydillo, Ignacio Mena, Xin Yin, Laura Martin-Sancho, Nevan J. Krogan, Sumit K. Chanda, Michael Schotsaert, Richard W. Wozniak, Yi Ren, Brad R. Rosenberg, Beatriz M. A. Fontoura, and Adolfo García-Sastre
PNAS. 2020. DOI: 10.1073/pnas.2016650117