Movie S5.

High [Ca²⁺]_e and acidic pH_e values induced the elevation in [Ca²⁺]_i, cell membrane permeability, KHG elimination, and DNA degradation of an isolated SG1 cell in vitro A representative time-lapse movie of an isolated SG1 cell undergoing cell death. An isolated SG1 cell was treated with acidic extracellular pH (pH_e5.60) under high [Ca²⁺] (1 mM) levels in vitro. Images were obtained using the BioStation IM-Q (NIKON) at 5-min intervals for 18 h 5 min. Merged snapshot image of phase-contrast and EGFP (green) before replacing the acidic medium is shown (left). Merged movie of phase-contrast, Rhod4-AM ([Ca²⁺]_i; magenta), and DRAQ7 (cell membrane permeability and DNA degradation; cyan) is shown (center). Images on the extreme right are the magnified versions of the image marked using a yellow box in the middle image. Arrows indicate EGFP-positive SG1 cells. Scale bar: 10 μm.