Movie S4.

Pico-injection Proof-Of-Concept. Here, in order to confirm pico-injection occurred uniformly, an aqueous phase was injected into water droplets. As the droplets pass through the injection chamber, a voltage is applied to merge the aqueous channel and the droplet, while the channel post-chamber helps to mix the contents of the droplets themselves. After injection, image quantification was performed to confirm uniformity (supplementary figure 4).

Sorting for secreted molecule production using a biosensor-in-microdroplet approach

Emily K. Bowman, James M. Wagner, Shuo-Fu Yuan, Matthew Deaner, Claire M. Palmer, Simon D’Oelsnitz, Lauren Cordova, Xin Li, Frank F. Craig, and Hal S. Alper

PNAS. 2021. 118:None-None DOI: 10.1073/pnas.2106818118