Time-lapse imaging of oocytes during bisphenol exposure. Metaphase II (MII) oocytes with an assembled meiotic spindle were cultured for 4 h in media alone (top, control) or media supplemented with 50 mg/mL of BPA (middle) or BPF (bottom). Time-lapse (15 min intervals) movie of laser scanning confocal Z-stack reconstruction (every 5 μm) of oocytes expressing H2B-RFP (red) and MAP-4-EGFP (green) that label chromosomes and spindle microtubules, respectively. This video (http://movie-usa.glencoesoftware.com/video/10.1530/REP-19-0494/video-1) is available from the online version of the article at https://doi.org/10.1530/REP-19-0494.