Computer-assisted sperm analysis (CASA) of sperm from the cauda epididymis of Zdhhc5 ^+/+ male mice. The video shows that sperm from WT mice exhibit normal physiological characteristics: sperm count, total motility, and progressive motility all conform to the typical reference range of healthy male mice, with most sperm displaying active movement and regular forward-moving trajectories. This video (https://movie-usa.glencoesoftware.com/video/10.1530/REP-25-0310/video-1) is available from the online version of this article at https://doi.org/10.1530/REP-25-0310.