Computer-assisted sperm analysis (CASA) of sperm from the cauda epididymis of Zdhhc5 ^−/− male mice. Compared with WT mice, the video clearly demonstrates significant abnormalities in sperm parameters of Zdhhc5 ^−/− mice: sperm count is markedly reduced, and both total motility and progressive motility are obviously decreased. Specific manifestations include fewer observable sperm, reduced movement frequency of individual sperm, and a significant increase in non-progressive or immotile sperm. This video (https://movie-usa.glencoesoftware.com/video/10.1530/REP-25-0310/video-2) is available from the online version of this article at https://doi.org/10.1530/REP-25-0310.