Fluorescence live imaging and tracking of embryos injected with an autophagic flux probe. Related to Fig. 2A. Fluorescence live imaging of IVF embryos at 24 h after insemination. From left to right, BF, Merge of GFP-LC3 and RFP-LC3ΔG, and tracking regions identified from the acquired images. Scale bar indicates 20 μm. This video (https://movie-usa.glencoesoftware.com/video/10.1530/REP-25-0338/video-1) is available from the online version of the article at (https://doi.org/10.1530/REP-25-0338).